ANTI-CHITINASE-3-LIKE PROTEIN 1 (YKL-40)

Analysis Note

ControlMG-63 conditioned media

Application

Research Sub CategoryDevelopmental Signaling

Research CategoryCell Structure

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Chitinase-3-like protein 1 (YKL-40) in U87 cells (This protein blocks tumor angiogenesis tube formation and progression (Faibish, M., et al. (2011). Mol Cancer Ther. 10(5):742-751.)).

Activity Assay Analysis: A representative lot from an independent laboratory neutralized Chitinase-3-like protein 1 in a neutralization assay (Faibish, M., et al. (2011). Mol Cancer Ther. 10(5):742-751.).

This Anti-Chitinase-3-like protein 1 (YKL-40) Antibody, clone mAY is validated for use in Western Blotting, IHC, Activity Assay for the detection of Chitinase-3-like protein 1 (YKL-40).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

YKL-40 (Chitinase-3-like protein 1; human cartilage glycoprotein 39) is a secreted glycoprotein belonging to the glycosyl hydrolase 18 (GH18) family. It is found primarily in chondrocytes, activated macrophages, and hepatocytes. The precise biological function of YKL-40 is still unknown; however, recent studies suggest that YKL-40 may play a role in cell differentiation and inflammation. YKL-40 is elevated in the sera of patients with inflammatory diseases or cancer, and it is currently being investigated as a potential biomarker for these conditions.

Immunogen

Recombinant protein corresponding to human Chitinase-3-like protein 1 (YKL-40).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Format: Purified

Quality

Evaluated by Western Blot in MG-63 conditioned media.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Chitinase-3-like protein 1 (YKL-40) in 10 µg of MG-63 conditioned media (This protein blocks tumor angiogenesis tube formation and progression (Faibish, M., et al. (2011). Mol Cancer Ther. 10(5):742-751.)).

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~42 kDa observed